{"id":489,"date":"2024-03-10T13:41:49","date_gmt":"2024-03-10T13:41:49","guid":{"rendered":"https:\/\/pri.tum.ac.ke\/?page_id=489"},"modified":"2024-03-10T13:41:50","modified_gmt":"2024-03-10T13:41:50","slug":"optimization-of-growth-conditions-for-production-of-organophosphorous-phosphatase-enzymes-in-three-bacteria-species-isolated-from-dairy-farms-in-kenya","status":"publish","type":"page","link":"https:\/\/pri.tum.ac.ke\/?page_id=489","title":{"rendered":"Optimization Of Growth Conditions For Production Of Organophosphorous Phosphatase Enzymes In Three Bacteria Species Isolated From Dairy Farms In Kenya"},"content":{"rendered":"\n<p>Tom Felix Juma, Gicharu Gibson Kamau, Gona Makenzi Nzaro, Chimbevo Lenny Mwagandi, Mjomba Fredrick Mwamburi, Amana Mzee Juma<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><strong>ABSTRACT<\/strong><\/h3>\n\n\n\n<p>Chlorfenvinphos is one of the Organophosphates largely produced around the world to control mites and ticks in dairy farming. However, it is toxic to human health through adverse effects on the nervous system and potential harm to other organ systems and have a long residual effect posing risks to both aquatic and terrestrial ecosystems. This study aimed at optimizing pH and temperature and Screening for chlorfenvinphos degrading organophosphorus phosphatase enzymes from three chlorpyrifos-degrading bacterial isolates, namely <em>Advenellakashmirensis,Micrococcusluteus,<\/em>and <em>Lysinibacillus<\/em> <em>sphaericus<\/em>. The bacteria were grown at varying pH and temperature. Results indicated different growth rates as indicated by optical density (OD) values taken at different pH and temperature. <em>Advenella kashmirensis <\/em>exhibited the highest growth while <em>Lysinibacillussphaericus<\/em>had the lowest. The optimal conditions for growth were determined to be at pH 7 and 35\u00b0C, resulting in an optical density (OD) of 0.132 for <em>Lysinibacillus sphaericus<\/em>. For <em>Advenella kashmirensis<\/em>, the optimum conditions were identified at pH 6 and 35\u00b0C, yielding an OD of 0.175. <em>Micrococcus luteus <\/em>demonstrated maximal growth at pH 7 and 35\u00b0C, attaining an OD of 0.106. When analyzed for crude protein concentration, <em>A. kashmirensis <\/em>exhibited the highest crude protein concentration (465.407\u00b131.820 \u03bcg\/mL) followed by <em>L. sphaericus <\/em>(303.556\u00b120.368 \u03bcg\/mL) and lastly M. luteus (292.074\u00b18.373 \u03bcg\/mL). <em>A. kashmirensis <\/em>exhibited highest crude protein concentration (465.407\u00b131.820 \u03bcg\/mL) and was significantly different at P &lt; 0.05. This, suggest that <em>Advenellakashmirensis<\/em>is suitable for applications requiring high concentrations of total proteins compared to the two. Enzyme activity increased after purification steps particularly through ammonium sulfate precipitation and gel filtration with <em>A. kashmirensis <\/em>exhibiting the highest enzyme activity after gel filtration (19.761\u00b10.818 \u00b5Mol\/Min in Fraction I), followed by <em>M.luteus<\/em>(13.772\u00b10.979 \u00b5Mol\/Min in Fraction I) and <em>L.sphaericus<\/em>(11.808\u00b10.181 \u00b5Mol\/Min in Fraction I) among the three <em>Advenella kashmirensis <\/em>had the highest activity (19.761\u00b10.818 \u00b5Mol\/Min and was significantly different at P &lt; 0.05<em>.<\/em>Therefore A. <em>kashmirensis <\/em>can be applied for bioremediation applications. However, future studies should explore the potential applications of these organophosphorus phosphatase enzymes obtained from <em>A. kashmirensis, M. luteus, <\/em>and <em>L. sphaericus <\/em>in chlorfenvinphos degradation for use in bioremediation, this would provide practical implementation.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tom Felix Juma, Gicharu Gibson Kamau, Gona Makenzi Nzaro, Chimbevo Lenny Mwagandi, Mjomba Fredrick Mwamburi, Amana Mzee Juma ABSTRACT Chlorfenvinphos is one of the Organophosphates [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":109,"menu_order":8,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-489","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/pri.tum.ac.ke\/index.php?rest_route=\/wp\/v2\/pages\/489","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pri.tum.ac.ke\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pri.tum.ac.ke\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pri.tum.ac.ke\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/pri.tum.ac.ke\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=489"}],"version-history":[{"count":1,"href":"https:\/\/pri.tum.ac.ke\/index.php?rest_route=\/wp\/v2\/pages\/489\/revisions"}],"predecessor-version":[{"id":490,"href":"https:\/\/pri.tum.ac.ke\/index.php?rest_route=\/wp\/v2\/pages\/489\/revisions\/490"}],"up":[{"embeddable":true,"href":"https:\/\/pri.tum.ac.ke\/index.php?rest_route=\/wp\/v2\/pages\/109"}],"wp:attachment":[{"href":"https:\/\/pri.tum.ac.ke\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=489"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}